WSU IEHS Faculty Profile for Raymond F. Novak


	Raymond F. Novak, Ph.D.
	Wayne State University Director, Institute of Environmental Health Sciences Director, EHS Center for Molecular & Cellular Toxicology with Human Applications Professor, Dept. of Pharmacology
	Tel.	(313) 577- 0100
	Fax.	(313) 577- 0082
	Email:	r.novak@wayne.edu
	Address:	Institute of Environmental Health Sciences Wayne State University 2727 Second Avenue, Room 4000 Detroit, MI 48201-2654

Research Interests:
Our research focuses on the role of diabetes in altering the expression of cytochromes P450 (CYP2E1) and other enzymes involved in xenobiotic metabolism, oxidative stress and cellular protection in primary cultured hepatocytes at the level of transcription and translation, and the signaling pathways and components involved in regulating expression. Global gene expression is accomplished using microarray analysis and expression profiles, gene ontologies and signaling pathway analysis is performed in conjunction with intracellular signaling events. Our laboratory also examines the effects of altered cell signaling on human breast cancer development. The ability of various endogenous and exogenous compounds and signaling components which alter the association of cell adhesion molecules that ultimately affect cell morphology, as well as cell cycle regulation, proliferation and apoptosis, is being studied. The various protein and gene markers that contribute to the development of Proliferative Breast Disease (PBD), a risk factor for tumorigenesis, is being examined using proteomic and microarray gene expression technologies.

Select Publications:
L.C. Overton, A. Hudder, and R.F. Novak (2007) “CYP2E1: Distribution, Xenobiotic Metabolism, and Regulation of Gene and Protein Expression: Role of Intracellular Signaling,” In: Cytochrome P450: Role in the Metabolism and Toxicity of Drugs and Other Xenobiotics (ed. C. Ionnides) Issues in Toxicology, Royal Society of Chemistry, London, UK., In Press. Hudder A, Novak RF. miRNAs: effectors of environmental influences on gene expression and disease. Toxcol. Sci. 2008, Feb 16; [Epub ahead of print] In Press. Kim SK, Novak RF. The role of intracellular signaling in insulin-mediated regulation of drug metabolizing enzyme gene and protein expression. Pharmacol Ther. 2007 Jan;113(1):88-120. PMID: 17097148 Kim SK, Abdelmegeed MA, Novak RF. The mitogen-activated protein kinase kinase (mek) inhibitor PD98059 elevates primary cultured rat hepatocyte glutathione levels independent of inhibiting mek. Drug Metab Dispos. 2006 Apr;34(4):683-9.PMID: 16443668. Dombkowski AA, Cukovic D, Novak RF. Secretome analysis of microarray data reveals extracellular events associated with proliferative potential in a cell line model of breast disease. Cancer Lett. 2006 Sep 8;241(1):49-58. Epub 2005 Nov 17. PMID: 16298039 Kim SK, Abdelmegeed MA, Novak RF. Identification of the Insulin Signaling Cascade in the Regulation of Alpha-Class Glutathione S-Transferase Expression in Primary Cultured Rat Hepatocytes. J Pharmacol Exp Ther. 2005 Nov 17; PMID: 16293713. Kim SK, Woodcroft KJ, Oh SJ, Abdelmegeed MA, Novak RF. Role of mechanical and redox stress in activation of mitogen-activated protein kinases in primary cultured rat hepatocytes. Biochem Pharmacol. 2005 Dec 5;70(12):1785-95. PMID: 16242670. Kim SK, Woodcroft KJ, Khodadadeh SS, Novak RF. Insulin signaling regulates gamma-glutamylcysteine ligase catalytic subunit expression in primary cultured rat hepatocytes. J Pharmacol Exp Ther. 2004 Oct;311(1):99-108. PMID: 15169830 Abdelmegeed MA, Kim SK, Woodcroft KJ, Novak RF. Acetoacetate activation of extracellular signal-regulated kinase 1/2 and p38 mitogen-activated protein kinase in primary cultured rat hepatocytes: role of oxidative stress. J Pharmacol Exp Ther. 2004 Aug;310(2):728-36. PMID: 15051799 Dombkowski AA, Thibodeau BJ, Starcevic SL, Novak RF. Gene-specific dye bias in microarray reference designs. FEBS Lett. 2004 Feb 27;560(1-3):120-4. PMID: 14988009 Kim SK, Woodcroft KJ, Kim SG, Novak RF. Insulin and glucagon signaling in regulation of microsomal epoxide hydrolase expression in primary cultured rat hepatocytes. Drug Metab Dispos. 2003 Oct;31(10):1260-8. PMID: 12975336 Starcevic SL, Diotte NM, Zukowski KL, Cameron MJ, Novak RF. Oxidative DNA damage and repair in a cell lineage model of human proliferative breast disease (PBD). Toxicol Sci. 2003 Sep;75(1):74-81. PMID: 12805649 Kim SK, Woodcroft KJ, Novak RF. Insulin and glucagon regulation of glutathione S-transferase expression in primary cultured rat hepatocytes. J Pharmacol Exp Ther. 2003 Apr;305(1):353-61. PMID: 12649389 Kang KW, Novak RF, Lee CH, Kim SG. Induction of microsomal epoxide hydrolase by sulfur amino acid deprivation via the pathway of C-Jun N-terminal kinase and its extracellular exposure during cell death. Free Radic Biol Med. 2002 May 15;32(10):1017-32. PMID: 12008117 Woodcroft KJ, Hafner MS, Novak RF. Insulin signaling in the transcriptional and posttranscriptional regulation of CYP2E1 expression. Hepatology. 2002 Feb;35(2):263-73. PMID: 11826398 Starcevic SL, Bortolin S, Woodcroft KJ, Novak RF. Kepone (chlordecone) disrupts adherens junctions in human breast epithelial cells cultured on matrigel. In Vivo. 2001 Jul-Aug;15(4):289-94. PMID: 11695219 Starcevic SL, Elferink C, Novak RF. Progressive resistance to apoptosis in a cell lineage model of human proliferative breast disease. J Natl Cancer Inst. 2001 May 16;93(10):776-82. PMID: 11353788